Direct RNA sequencing

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Product introduction
Common problem
Classic case
Result display

Direct RNA sequencing is based on the Nanopore sequencing platform, without reverse transcription, no amplification, direct reading of full-length transcripts, no sequencing preference, detection of RNA molecule methylation (m6A and m5C) and other modification sites; accurate analysis of variable splicing, fusion of genes and identification of new isomers; in addition, can also make relatively accurate estimates of Poly(A) tail length, restore true RNA features.
The main analysis content
Poly(A) Length Analysis (Unique)
RNA modification analysis (m6A and m5C)
Isoform expression quantification
Variable shear analysis
Fusion gene analysis

A selection of high-IF articles in recent years

Journals	IF	Time	Article title
Genome Biology	13.583	2022.01.17	DENA: training an authentic neural network model using Nanopore sequencing data of Arabidopsis transcripts for detection and quantification of N6-methyladenosine on RNA
Advanced Science	16.806	2022.01.05	FIONA1-Mediated m6A Modification Regulates the Floral Transition in Arabidopsis
Genome Biology	13.583	2021.12.06	Interferon inducible pseudouridine modification in human mRNA by quantitative nanopore profiling
Nucleic Acids Research	16.971	2021.08.18	TERA-Seq: true end-to-end sequencing of native RNA molecules for transcriptome characterization
Nature Communications	14.919	2021.08.16	Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae
Nature Biotechnology	54.908	2021.07.19	Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore
Genome Biology	13.583	2021.06.28	Direct long-read RNA sequencing identifies a subset of questionable exitrons likely arising from reverse transcription artifacts
Nature Biotechnology	54.908	2021.05.13	Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing. Nat Biotechnol.
Cell Report	8.109	2021.04.30	Cytoplasmic polyadenylation by TENT5A is required for proper bone formation
Genome Biology	13.583	2021.01.07	Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing