CircRNA sequencing

TECHNOLOGY SERVICES

Product introduction
Common problem
Classic case
Result display

Circular RNA (circRNA) is a new class of non-coding RNAs that are widely present in plants and animals. By enriching circRNA by removing rRNA and linear RNA by strand-specific library building method, based on high-throughput sequencing, a large amount of circRNA information can be obtained, and the structure and target target of cirRNA can be predicted through bioinformatics analysis

Product features

Identify circRNA transcripts by circRNA cyclic splicing and analyze the source of circRNA.

The expression of circRNA is generally tissue specific, and by estimating the amount of circRNA expression in all samples, it is possible to understand the expression pattern of circRNA in the sample, and the correlation between fractal samples in the expression pattern.

Between the treatment group and the control group, the differentially expressed circRNA was analyzed, and the relationship between the differential circRNA and the phenotypic difference was speculated.
KEGG/GO enrichment of source genes

Transcripts of genes that exist under certain conditions form circRNA instead of mRNA. GO/KEGG enrichment analysis of the source genes of differential circRNAs is helpful in analyzing the differential phenotype and circRNA function.

Studies have found the presence of miRNAs binding locations on some circRNA sequences, which can recruit miRNAs and participate in transcriptional regulation.