Nanopore sequencing technology, the core technology of Oxford nanopore, is recognized as a major direction of sequencing development in the future: different from previous technologies, it reads bases not by detecting light, fluorescence signals or pH, but based on electrical signals. Nanopores are embedded in the synthetic membrane and immersed in electrophysiological solution. When DNA or RNA molecules pass through the nanopore, they will interfere with the current and cause the change of current signal. The signal is analyzed in real time to determine the base sequence passing through the pore.
Characteristics of nanopore sequencing
Super long reading length:
L DNA angle can simplify the assembly, deal with highly repetitive genome and improve the index of genome assembly;
L full length transcripts can be obtained from the perspective of RNA for isomer and variable shear analysis.
Direct sequencing:
L sequencing the original DNA and RNA strand directly without PCR and other means.
L avoid amplified base preference;
L base modification information (5mC, 6mA, etc.) can be retained.