Nanopore DNA methylation_Animal and plant resequencing_Services

TECHNOLOGY SERVICES

Animal and plant genomes
Microbial genome
Transcriptional regulation
Microbial diversity
Animal and plant resequencing
Proteomics and metabolomics
Pure sequencing
- Third generation database sequencing
- Second generation database building sequencing

Product introduction
Common problem
Classic case
Result display

Product introduction

DNA methylation, a form of epigenetic modification that does not alter the DNA sequence, is currently the most studied form of DNA methylation, mainly for 6mA and 5mC. Nanopore sequencing detects both 6mA and 5mC directly by sequencing without the need for bisulfate conversion or immunoprecipitation enrichment experiments.

Advantages of Nanopore DNA methylation

Sequence distribution of methylation sites
Methylation levels in different regions

Genetic Imprinting（Gigante et al, 2019）

Characteristics of various methylation detection techniques（Quentin Gouil and Andrew Keniry, 2019）

Publications

Gouil Q , Keniry A . Latest techniques to study DNA methylation. Essays in Biochemistry, 2019.
Scott, Gigante, Quentin, et al. Using long-read sequencing to detect imprinted DNA methylation. Nucleic Acids Research, 2019.