Product introduction

T2T (Telomerase-to-telomere) or gapless genome is a high-quality genome with high genomic accuracy, continuity and integrity. With the development of three-generation sequencing technology, especially the combination of high-continuity ONT ultra-long sequencing and high-accuracy HIFI sequencing, the problem of difficult assembly of centromere or highly repetitive regions have been overcome, and the continuity and integrity of chromosomes have been greatly improved, leading to the near-0 gap era of genome assembly.
 
The assembly of the T2T genome facilitates the in-depth research of highly repetitive sequence regions or highly repetitive structures in the genome, and provides an opportunity for the research of the variant characteristics of centromere regions or unknown highly repetitive regions.
 
As one of the few service providers in China that can continuously produce high-quality ONT ultra-long data, Wuhan Benagen Technology Co., Ltd. has long been committed to the research and development of the yield and quality of ONT sequencing data and the quality of ONT ultra-long genome assembly. At present, it has achieved the output of ONT ultra-long single cell with over 10G sequencing reads N50>100Kb data, as well as the assembly of ultra-long reads of several genomes.
 
1. 完成着丝粒组装     Completion of centromere assembly
2. 完成端粒组装       Completion of telomere assembly
3. Gap-free，基因组完整度更高    Gap-free and higher genome integrity
4. 重复区域完美组装     Perfect assembly of segmental duplications 

Classic Cases

Reference case 1：The structure, function and evolution of acomplete human chromosome 8

Journal: Nature（IF=49.962）

Assembly strategy：ONT ultra-long （20x）+HiFi (32.4x)

Reference: Logsdon GA et al. The structure, function and evolution of a complete human chromosome 8. Nature. 2021. 

doi: 10.1038/s41586-021-03420-7.
 
Reference case 2：High-quality Arabidopsis thaliana genome assembly with Nanopore and HiFi long reads

Journal: Genomics Proteomics Bioinformatics  （IF= 7.691）

Assembly strategy：ONT ultra-long > 50kb （177x）+ HiFi （153x）

Reference: Wang B, et al. High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads. Genomics Proteomics Bioinformatics. 2021.

doi: 10.1016/j.gpb.2021.08.003.