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Product introduction

The wild-type plants were mutated by EMS to obtain the mutant type, and the mutant plants were crossed with the wild-type after multiple generations of self-fertilization to obtain F1, and F1 was self-fertilized to obtain F2, and F2 showed segregation of traits.
Several strains of F2 were selected for sequencing in a mixed pool of individuals with phenotypic inconsistency with the wild type, while the wild type sequencing was used as the reference gene sequence.
SNP-Index (the proportion of reads different from the reference sequence to the total reads) significantly higher than the desired SNP site was used as a candidate site to cause the mutation.


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