Introduction

Pore-C is a novel technology that combines chromatin conformation capture (3C) with Nanopore long-read sequencing to detect multi-locus interactions and associated methylation modifications in species genomes. It offers a simpler workflow without the need for biotin labeling or PCR amplification, capable of detecting complex GC-rich regions and repetitive genomic regions while preserving epigenetic modification information.

The Pore-C technology can reveal more interaction information, distinguish allelic genes more easily, effectively improve the assembly level of animal and plant genomes, correct misassemblies, and provide conditions for in-depth analysis of three-dimensional genome spatial structure characteristics and chromatin multiplex interactions research.

Applications

• Improving assembly contiguity

• 3D genome research

• DNA methylation modification and chromosome structure relationship research

Highlights

• Exhibits higher efficiency and fidelity

• The absence of an amplification step that is required in other chromatin conformation capture methods

• Bisulfite conversion (as is required in Methyl-HiC) is not required when detecting DNA methylation

• Capable of detecting a greater number of higher-order interactions

Sequencing workflow

Data Delivery File Foemats

Fastq and Fast5 Data Files

Data Delivery Standard

Sequencing yield display

What‘s the best choice?