Introduction

Nanopore direct RNA sequencing (DRS) technology enables the sequencing of continuous native RNA strands, thereby allowing for the detection and documentation of nucleotide modifications and poly(A) tails without requiring additional chemistry steps.

Applications

• Research on RNA Structure and Function

• Disease Diagnosis and Treatment Studies

• Drug Development and Clinical Research

• Environmental Response and Stress Adaptation Studies

Highlights

• Detect RNA modifications (m5C, m6A, ψ, etc.) at single-based level

• Each read has a poly(A) length

• Direct sequencing of RNA molecules without reverse transcription avoids the introduction of false-positive transcripts

Library Construction workflow

Overview of the nanopore RNA-sequencing methodology

(Depledge et al., 2019, DOI:10.1038/s41467-019-08734-9)

Sample Requirements

Sample storage: The sample should be stored at -80°C. Avoid repeated freezing and thawing.

Data Delivery File Formats

Fastq and Fast5 Data Files

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